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Protein_Domain
Part:BBa_K4633001:Design
Designed by: Neta Segal Group: iGEM23_Technion-Israel (2023-09-07)
AmyA - signal peptide for Lactobucilli
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The testing of the AmyA signal peptide was conducted in Lactobacillus crispatus ATCC 33802, utilizing the pMG36E plasmid sourced from AddGene.
Source
The amino acid sequence of the AmyA signal peptide was originally documented in the work of Tran, A. M. et al. [1]. It was reverse-translated to align with the genome of Lactococcus lactis using Benchling.
References
[1] Tran, A. M. et al. Efficient Secretion and Recombinant Production of a Lactobacillal α-amylase in Lactiplantibacillus plantarum WCFS1: Analysis and Comparison of the Secretion Using Different Signal Peptides. Front. Microbiol. 12, 689413 (2021).