Protein_Domain

Part:BBa_K4633001:Design

Designed by: Neta Segal   Group: iGEM23_Technion-Israel   (2023-09-07)


AmyA - signal peptide for Lactobucilli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The testing of the AmyA signal peptide was conducted in Lactobacillus crispatus ATCC 33802, utilizing the pMG36E plasmid sourced from AddGene.


Source

The amino acid sequence of the AmyA signal peptide was originally documented in the work of Tran, A. M. et al. [1]. It was reverse-translated to align with the genome of Lactococcus lactis using Benchling.


References

[1] Tran, A. M. et al. Efficient Secretion and Recombinant Production of a Lactobacillal α-amylase in Lactiplantibacillus plantarum WCFS1: Analysis and Comparison of the Secretion Using Different Signal Peptides. Front. Microbiol. 12, 689413 (2021).